Coding

Part:BBa_K2201410

Designed by: Olga Schmidt   Group: iGEM17_Bielefeld-CeBiTec   (2017-10-27)


Kanamycin composite part containing amber codons


This part contains the coding sequence of the kanamycin resistence of pSB1K3 with two amber codons under the control of an Anderson promoter (BBa_J23100). The kanamycin resistence gene allows microorganisms to grow in the presence of the antibiotic agent.

Usage and Biology

This composite part contains the kanamycin resistence gene with amber codons at the amino acid poistions S133Am and S154Am. The sequence is reverse. This part can be used for a possible positive selection plasmid.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 964
    Illegal NheI site found at 987
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None